ASTM E2315
ASTM E2315, or Time Kill studies, evaluate the decrease of a microbial population of test organisms after being exposed to test products and materials containing antimicrobials. This test assesses in vitro reduction of a population of aerobic microorganisms within a specified period when antimicrobials are present. Accugen Laboratories, Inc. offers various testing conditions to be requested for research and development purposes.
A culture of microorganism is added to a tested disinfectant and observed after a specified time in incubation. After the time passes, the solution is neutralized and plated in serial dilutions. The presence of microorganisms is determined before and after testing to conclude a percent reduction. This procedure allows for an analysis of tests done under varying environments and testing conditions.
Products that are commonly tested using this method:
- Soaps
- Detergents
- Alcohols
- Chlorine
- Disinfectants
- Hand Sanitizers
- Wipes
Microorganisms tested against in an ASTM E2315 Time Kill study:
- Staphylococcus aureus
- Salmonella cholerasuis
- Pseudomonas aeruginosa
- Escherichia coli
- Aspergillus niger
- Trichophyton mentagrophytes
- Other microorganisms can be requested
Sample Size/Test Duration:
At least 30 grams of sample are required to perform ASTM E2315 testing. Smaller sample sizes can be accommodated if more cannot be provided. Testing occurs in predetermined intervals depending on each sample’s requirements, but the test duration is usually 1-3 days. Varying time intervals may be requested.